The International Association of Providers in AIDS Care (IAPAC), joined by the African Society for Laboratory Medicine (ASLM) and other partners, as well as global HIV and health experts, have developed global recommendations to expedite the introduction, uptake, and scale-up of HIV self-testing (HIVST) as part of the Fast-Track Cities initiative. Tailored to the specific needs of cities, these recommendations have been shaped to support and facilitate rapid implementation of 2016 World Health Organization (WHO) guidelines recommending that HIVST be offered as an additional approach to traditional HIV testing services. These global recommendations are intended for local decision-makers, policy-makers at the national level whose actions affect the scope of action in cities, international donors and technical agencies, city-based clinicians (both in the private and public sectors), and civil society and other stakeholders in Fast-Track Cities.